胶原酶IV型/IV型胶原酶/Collagenase Type IV,0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
- 产品编号：C0126
- 中文名称：胶原酶IV型/IV型胶原酶/Collagenase Type IV
- 英文名称：Collagenase from Clostridium histolyticum
- 英文别名:Clostridiopeptidase A
- CAS号:9001-12-1
- 纯度:0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
- EC号:232-582-9
性能描述
| suitability | release of physiologically active rat hepatocytes tested |
| storage temp. | ?20°C |
Collagenase from Clostridium histolyticum
release of physiologically active rat hepatocytes tested, Type IV, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
Analysis Note
Also contains clostripain, neutral protease and tryptic activities.
Application
Collagenase products offered by Sigma-Aldrich are prepared from culture supernatants of C. histolyticum. Most preparations contain collagenase, non-specifc protease, and clostripain activities. These enzymes act synergistically to degrade collagen and other intercellular material. Crude Collagenases are suitable for isolation of cells from many types of animal tissue. All are evaluated for their enzymatic activities. The optimal concentration for use and digestion time of tissue needs to be empirically determined. In addition, Sigma-Aldrich also offers collagenases which are tested for suitability for release of cells from specified tissues (release of rat pancreatic islets, rat epididymal adipocytes, and rat hepatocytes.
