碱性蛋白酶/细菌蛋白酶/Proteinase/Alkaline Protease,7.0-14.0 units/mg solid
- 产品编号：P0112
- 中文名称：碱性蛋白酶/细菌蛋白酶/Proteinase/Alkaline Protease
- 英文名称：Proteinase, bacterial
- 英文别名:Protease from Bacillus licheniformis, Proteinase from Bacillus licheniformis, Subtilo peptidase A
- CAS号:9014-01-1
- 纯度:7.0-14.0 units/mg solid
- EC号:232-752-2
- MDL号:MFCD00132092
性能描述
| t类型 | Type XXIV |
| 性状 | lyophilized powder |
| activity | ≤0.05 Kunitz units/mg solid RNase |
| ≤5.0 Kunitz units/mg solid DNase |
| purified by | crystallization |
| 保存温度 | ?20°C |
Application
Proteinase is an enzyme used to break down proteins by hydrolyzing peptide bonds. Proteinase is used to degrade proteins, to study proteinase inhibitors and to study thermal inactivation kinetics. Proteinase is used in nucleic acid isolation procedures in incubations. It is used to study proteinase-activated receptors, such as the transducers of proteinase-mediated signaling in inflammation and the immune response. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1.
Biochem/physiol Actions
Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .
碱性蛋白酶是经细菌原生质体诱变方法造育的2709枯草杆微生物通过深层发酵、提取及精制而成的一种蛋白水解酶，属于一种丝氨酸脆外高碱性蛋白酶，它能水解蛋白质分子肽链生成多肽或氨基酸，具有较强的分解蛋白质的能力。
